MO335URINARY CYTOKINES REFLECT THE ONGOING RENAL INFLAMMATION IN THE DIAGNOSTIC OF ACUTE TUBULOINTERSTITIAL NEPHRITIS: RESULTS OF A MULTIPLEX BEAD-BASED ASSAY ASSESSMENT

Abstract Background and Aims Acute tubulointerstitial nephritis (ATIN) diagnostic lays on the kidney biopsy given absence of non-invasive biomarkers for disease demonstration follow-up. The aim this study was to evaluate accuracy ten urinary inflammatory-related cytokines in ATIN its clinical distinction from acute tubular necrosis (ATN). Method Observational prospective including 21 12 ATN patients, 6 healthy controls. We determined levels 10 inflammation-related using a multiplex bead-based Luminex assay. registered clinical, analytical histological data medical records. Results Urinary I-TAC/CXCL11, CXCL10, IL-6, TNFα MCP-1 were higher compared In contrast, controls exhibited EGF patients. Follow-up samples available 11/21 patients showed significant decrease MIG/CXCL9 CXCL10 levels. IL-6 significantly with I-TAC/CXCL11 as best discriminatory biomarker based AUC ROC curve likelihood ratio. combinatory model three increased sensitivity individual ATIN/ATN but results obtained when blood eosinophil count leukocyturia added model. found positive correlation extent infiltrate biopsies concentration MIG/CXCL9, IL17, IFNα, MCP1 EGF, indicating potential renal source Conclusion cytokine controls, decline after treatment grade inflammatory allows us propose IL6 candidate disease.